SMC1A antibody (pSer966)

Catalog No. ABIN7455211

The Rabbit Polyclonal anti-SMC1A antibody has been validated for WB, IP, IHC (fp) and IF/ICC. It is suitable to detect SMC1A in samples from Human.

Quick Overview for SMC1A antibody (pSer966) (ABIN7455211)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunofluorescence (fixed cells) (IF/ICC)

Product Details anti-SMC1A Antibody

Binding Specificity: pSer966

Purpose: Rabbit anti-Phospho SMC1 (S966) Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: surrounding serine 966

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:500 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IHC: 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1

Background: Background: Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.

Gene ID: 8243

NCBI Accession: NP_006297

UniProt: Q14683

Pathways: Stem Cell Maintenance