KIF14 antibody (C-Term)

Catalog No. ABIN7455214

The Rabbit Polyclonal anti-KIF14 antibody has been validated for WB, IP, IF/ICC and IHC (fp). It is suitable to detect KIF14 in samples from Human.

Quick Overview for KIF14 antibody (C-Term) (ABIN7455214)

Target: KIF14 (Kinesin Family Member 14 (KIF14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF14 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-KIF14 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-KIF14 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 1600 and C-term

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:200 - 1:1,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IHC: 1:200 to 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for KIF14

Target: KIF14 (Kinesin Family Member 14 (KIF14))

Alternative Name: KIF14

Background: Background: The kinesin superfamily of proteins consists of over forty KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. KIF14 silencing experiments suggest that KIF14 plays a multi-functional role in mitosis and cytokinesis. KIF14 has also been identified as a candidate oncogene whose overexpression may lead to genomic instability and act as a reporter of poor-prognosis in breast cancer.

Gene ID: 9928

NCBI Accession: NP_055690

UniProt: Q15058