Recombinant PD-L1 antibody (C-Term)

Catalog No. ABIN7455244

The Rabbit Monoclonal anti-PD-L1 antibody has been validated for WB, FACS, IHC, IP, IF (p), IF/ICC and mIF. It is suitable to detect PD-L1 in samples from Human.

Quick Overview for Recombinant PD-L1 antibody (C-Term) (ABIN7455244)

Target: PD-L1 (CD274 (PD-L1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (fixed cells) (IF/ICC), Multiplex Immunofluorescence (mIF)

Clone: BLR020E

Product Details anti-PD-L1 Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-PD-L1 Recombinant Monoclonal Antibody [BLR020E]

Purification: Purified

Immunogen: between AA 250 to 290 (C-term)

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

IHC-IF: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 20 μL/mg lysate

WB: 17:40:00

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 50 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1

Background: PD-L1 is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1 is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma [taken from NCBI Entrez Gene (Gene ID: 55193)].

Gene ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints