STAG2 antibody (N-Term)

Catalog No. ABIN7464162

This Rabbit Polyclonal antibody specifically detects STAG2 in WB, IF, IP, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for STAG2 antibody (N-Term) (ABIN7464162)

Target: STAG2 (Stromal Antigen 2 (STAG2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-STAG2 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SA2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for STAG2

Target: STAG2 (Stromal Antigen 2 (STAG2))

Alternative Name: stromal antigen 2

Background: Stromal antigen 2 , SA-2 , SA2 , SCC3B , bA517O1.1,The protein encoded by this gene is a subunit of the cohesin complex, which regulates the separation of sister chromatids during cell division. Targeted inactivation of this gene results in chromatid cohesion defects and aneuploidy, suggesting that genetic disruption of cohesin is a cause of aneuploidy in human cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

Molecular Weight: 141 kDa

Gene ID: 10735

UniProt: Q8N3U4

Pathways: Stem Cell Maintenance