PPIF antibody

Catalog No. ABIN7464912

This Rabbit Polyclonal antibody specifically detects PPIF in WB, IF, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PPIF antibody (ABIN7464912)

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-PPIF Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cyclophilin F. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: rat heart , mouse heart , A431 , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.67 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PPIF

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Alternative Name: peptidylprolyl isomerase F

Background: Peptidylprolyl isomerase F , CYP3 , CyP-M , Cyp-D , CypD,The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq]

Molecular Weight: 22 kDa

Gene ID: 10105

UniProt: P30405

Pathways: Proton Transport, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity