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ETV1 antibody

The Rabbit Polyclonal anti-ETV1 antibody has been validated for WB, IF, IHC (p), ICC and IHC (fro). It is suitable to detect ETV1 in samples from Human.
Catalog No. ABIN7464928

Quick Overview for ETV1 antibody (ABIN7464928)

Target

See all ETV1 Antibodies
ETV1 (Ets Variant 1 (ETV1))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This ETV1 antibody is un-conjugated

Application

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • Specificity

    This antibody was rasied against ER81 isoform 1. Based on sequence homology, this antibody can recognize most ER81 isoforms, but it may be unable to recognize isoform 4 according to the customer's feedback.

    Cross-Reactivity

    Human, Mouse, Rat

    Purification

    Purified by antigen-affinity chromatography.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human ER81. The exact sequence is proprietary.

    Isotype

    IgG
  • Application Notes

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Comment

    Positive Control: Rat2

    Validation: Orthogonal, Overexpression

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.83 mg/mL

    Buffer

    1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    ETV1 (Ets Variant 1 (ETV1))

    Alternative Name

    ETS variant 1

    Background

    ETS variant 1 , ER81,This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2016]

    Molecular Weight

    55 kDa

    Gene ID

    2115

    UniProt

    P50549
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