ETV1 antibody
Quick Overview for ETV1 antibody (ABIN7464928)
Target
See all ETV1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- This antibody was rasied against ER81 isoform 1. Based on sequence homology, this antibody can recognize most ER81 isoforms, but it may be unable to recognize isoform 4 according to the customer's feedback.
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Cross-Reactivity
- Human, Mouse, Rat
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Purification
- Purified by antigen-affinity chromatography.
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Immunogen
- Recombinant protein encompassing a sequence within the center region of human ER81. The exact sequence is proprietary.
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Isotype
- IgG
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Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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Comment
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Positive Control: Rat2
Validation: Orthogonal, Overexpression
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.83 mg/mL
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Buffer
- 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300
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Preservative
- ProClin
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Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- ETV1 (Ets Variant 1 (ETV1))
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Alternative Name
- ETS variant 1
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Background
- ETS variant 1 , ER81,This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2016]
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Molecular Weight
- 55 kDa
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Gene ID
- 2115
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UniProt
- P50549
Target
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