NOB1 antibody

Catalog No. ABIN7465472

This anti-NOB1 antibody is a Rabbit Polyclonal antibody detecting NOB1 in WB. Suitable for Human.

Quick Overview for NOB1 antibody (ABIN7465472)

Target: NOB1 (RNA-Binding Protein NOB1 (NOB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NOB1 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NOB1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , Jurkat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.01 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NOB1

Target: NOB1 (RNA-Binding Protein NOB1 (NOB1))

Alternative Name: NIN1 (RPN12) binding protein 1 homolog

Background: NIN1 (RPN12) binding protein 1 homolog , ART-4 , MST158 , MSTP158 , NOB1P , PSMD8BP1,In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM]

Molecular Weight: 47 kDa

Gene ID: 28987

UniProt: Q9ULX3

Pathways: Protein targeting to Nucleus