PATZ1 antibody (N-Term)

Catalog No. ABIN7465764

This Rabbit Polyclonal antibody specifically detects PATZ1 in WB, IF, IHC (p) and ICC. It exhibits reactivity toward Human.

Quick Overview for PATZ1 antibody (N-Term) (ABIN7465764)

Target: PATZ1 (ZNF278) (Zinc Finger Protein 278 (ZNF278))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PATZ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-PATZ1 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PATZ1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HCT116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.72 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PATZ1

Target: PATZ1 (ZNF278) (Zinc Finger Protein 278 (ZNF278))

Alternative Name: POZ/BTB and AT hook containing zinc finger 1

Background: POZ/BTB and AT hook containing zinc finger 1 , MAZR , PATZ , RIAZ , ZBTB19 , ZNF278 , ZSG , dJ400N23,The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1,22)(p36.1,q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq]

Molecular Weight: 74 kDa

Gene ID: 23598

UniProt: Q9HBE1