PFKP antibody

Catalog No. ABIN7465975

The Rabbit Polyclonal anti-PFKP antibody has been validated for WB. It is suitable to detect PFKP in samples from Human.

Quick Overview for PFKP antibody (ABIN7465975)

Target: PFKP (phosphofructokinase, Platelet (PFKP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PFKP Antibody

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PFKP. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa , Mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.29 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PFKP

Target: PFKP (phosphofructokinase, Platelet (PFKP))

Alternative Name: phosphofructokinase, platelet

Background: Phosphofructokinase, platelet , ATP-PFK , PFK-C , PFK-P , PFKF,The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM, MIM 610681) and liver (PFKL, MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM]

Molecular Weight: 86 kDa

Gene ID: 5214

UniProt: Q01813

Pathways: Warburg Effect