SIGLEC12 antibody

Catalog No. ABIN7466644

The Rabbit Polyclonal anti-SIGLEC12 antibody has been validated for WB and IHC (p). It is suitable to detect SIGLEC12 in samples from Human.

Quick Overview for SIGLEC12 antibody (ABIN7466644)

Target: SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIGLEC12 Antibody

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SIGLEC12. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.99 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SIGLEC12

Target: SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))

Alternative Name: sialic acid binding Ig like lectin 12 (gene/pseudogene)

Background: Sialic acid binding Ig like lectin 12 (gene/pseudogene) , S2V , SIGLECL1 , SLG , Siglec-XII,Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq]

Molecular Weight: 65 kDa

Gene ID: 89858

UniProt: Q96PQ1