QTRT1 antibody (Catalytic Subunit 1)

Catalog No. ABIN7467096

This Rabbit Polyclonal antibody specifically detects QTRT1 in WB, IHC (p), ICC and IF. It exhibits reactivity toward Human.

Quick Overview for QTRT1 antibody (Catalytic Subunit 1) (ABIN7467096)

Target: QTRT1 (Queuine tRNA-Ribosyltransferase 1 (QTRT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This QTRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-QTRT1 Antibody

Binding Specificity: Catalytic Subunit 1

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human QTRT1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NT2D1 , PC-3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.89 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for QTRT1

Target: QTRT1 (Queuine tRNA-Ribosyltransferase 1 (QTRT1))

Alternative Name: queuine tRNA-ribosyltransferase

Background: Queuine tRNA-ribosyltransferase catalytic subunit 1 , FP3235 , TGT , TGUT,tRNA-guanine transglycosylase (TGT, EC 2.4.2.29) synthesizes queuosine (Q), which is found in tRNAs that recognize NAU and NAC codons, encoding tyr, asn, asp, and his. Prokaryotic TGT is a single protein of 43 kD. In contrast, mammalian TGT appears to be a heterodimer consisting of a 60-kD subunit (USP14, MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande and Katze, 2001 [PubMed 11255023]).[supplied by OMIM]

Molecular Weight: 44 kDa

Gene ID: 81890

UniProt: Q9BXR0

Pathways: Ribonucleoside Biosynthetic Process