ATP6V1E1 antibody

Catalog No. ABIN7468013

Product Details anti-ATP6V1E1 Antibody

Target: ATP6V1E1 (ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E1 (ATP6V1E1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP6V1E1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse brain , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.96 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATP6V1E1

Target: ATP6V1E1 (ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E1 (ATP6V1E1))

Alternative Name: ATPase H+ transporting V1 subunit E1 (ATP6V1E1 Products)

Background:

Synonyms: ATPase H+ transporting V1 subunit E1 , ARCL2C , ATP6E , ATP6E2 , ATP6V1E , P31 , Vma4

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq]

Molecular Weight: 26 kDa

Gene ID: 529

UniProt: P36543

Pathways: Transition Metal Ion Homeostasis, Proton Transport