ATP6V1E1 antibody (Center)

Catalog No. ABIN2855175

The Rabbit Polyclonal anti-ATP6V1E1 antibody (ABIN2855175) specifically detects ATP6V1E1 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for ATP6V1E1 antibody (Center) (ABIN2855175)

Target: ATP6V1E1 (ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E1 (ATP6V1E1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1E1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V1E1 Antibody

Binding Specificity: Center

Cross-Reactivity: Chicken, Rat (Rattus), Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Chicken (85 %), Rat (98 %), Bovine (97 %), Xenopus tropicalis (83 %)

Characteristics: Rabbit Polyclonal antibody to ATP6V1E1 (ATPase, H+ transporting, lysosomal 31 kDa, V1 subunit E1)
ATP6V1E1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP6V1E1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , HepG2 , Molt-4 , Raji , mouse brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.17 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ATP6V1E1

Target: ATP6V1E1 (ATPase, H+ Transporting, Lysosomal 31kDa, V1 Subunit E1 (ATP6V1E1))

Alternative Name: ATP6V1E1

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome.

Molecular Weight: 26 kDa

Gene ID: 529

Pathways: Transition Metal Ion Homeostasis, Proton Transport