TNNT3 antibody (C-Term)

Catalog No. ABIN7468335

This Rabbit Polyclonal antibody specifically detects TNNT3 in WB. It exhibits reactivity toward Human.

Quick Overview for TNNT3 antibody (C-Term) (ABIN7468335)

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNT3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TNNT3 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Troponin T fast skeletal. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: human muscle , rat muscle , mouse muscle

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.19 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TNNT3

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Alternative Name: troponin T3, fast skeletal type

Background:

Synonyms: troponin T3, fast skeletal type , TNTF , beta-TnTF

Background: The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein, also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq]

Molecular Weight: 32 kDa

Gene ID: 7140

UniProt: P45378