LIN7A antibody

Catalog No. ABIN7468548

This Rabbit Polyclonal antibody specifically detects LIN7A in WB, IF, ICC, IHC (p) and IHC (wm). It exhibits reactivity toward Human.

Quick Overview for LIN7A antibody (ABIN7468548)

Target: LIN7A (Lin-7 Homolog A (LIN7A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIN7A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm))

Product Details anti-LIN7A Antibody

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human LIN7A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for LIN7A

Target: LIN7A (Lin-7 Homolog A (LIN7A))

Alternative Name: lin-7 homolog A, crumbs cell polarity complex component

Background:

Synonyms: lin-7 homolog A, crumbs cell polarity complex component , LIN-7A , LIN7 , MALS-1 , MALS1 , TIP-33 , VELI1

Background: Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.

Molecular Weight: 26 kDa

Gene ID: 8825

UniProt: O14910

Pathways: Synaptic Membrane