KMT5A antibody

Catalog No. ABIN7468629

This Rabbit Polyclonal antibody specifically detects KMT5A in ICC, IF, IHC (p) and WB. It exhibits reactivity toward Human.

Quick Overview for KMT5A antibody (ABIN7468629)

Target: KMT5A (N-Lysine Methyltransferase KMT5A (KMT5A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KMT5A antibody is un-conjugated

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Product Details anti-KMT5A Antibody

Cross-Reactivity: C. elegans, Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human SETD8. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for KMT5A

Target: KMT5A (N-Lysine Methyltransferase KMT5A (KMT5A))

Alternative Name: lysine methyltransferase 5A

Background:

Synonyms: lysine methyltransferase 5A , PR-Set7 , PR/SET07 , SET07 , SET8 , SETD8

Background: Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.

Molecular Weight: 43 kDa

Gene ID: 387893

UniProt: Q9NQR1