TSG101 antibody (AA 167-374)
Quick Overview for TSG101 antibody (AA 167-374) (ABIN7469107)
Target
See all TSG101 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Grade
Clone
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Binding Specificity
- AA 167-374
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Specificity
- Knockdown/Knockout validation was supported by references (PMID:27226592)
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Cross-Reactivity
- Dog, Hamster, Human, Monkey, Mouse, Pig, Rat, Zebrafish (Danio rerio)
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Purification
- Purified by antigen-affinity chromatography.
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Immunogen
- Amino acids 167-374 of TSG101 protein expressed in E. coli.
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Isotype
- IgG1
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Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:500-1:1000. IHC-P: 1:100-1:1000. FACS: 1:25-1:200. EM: 1:10. IHC: 1:100. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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Comment
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Positive Control: 293T , K562 , THP-1 , HL-60 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , NIH-3T3 , JC , BCL-1 Validation: KO/KD, Orthogonal, Overexpression, Recombinant antibody
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.8 mg/mL
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Buffer
- PBS, No Preservative
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Preservative
- Without preservative
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- TSG101 (Tumor Susceptibility Gene 101 (TSG101))
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Alternative Name
- tumor susceptibility 101
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Background
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Synonyms: tumor susceptibility 101 , TSG10 , VPS23
Background: The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]
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Molecular Weight
- 44 kDa
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Gene ID
- 7251
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UniProt
- Q99816
Target
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