ULK1 antibody (pSer758)

Catalog No. ABIN7469130

This Rabbit Polyclonal antibody specifically detects ULK1 in WB. It exhibits reactivity toward Human.

Quick Overview for ULK1 antibody (pSer758) (ABIN7469130)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ULK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ULK1 Antibody

Binding Specificity: pSer758

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser758 of human ULK1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7 ( starvation for 16 hr incubated in EBSS for 4 hr and 200 nM Rapamycin treatment for 0.5 hr) Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Alternative Name: unc-51 like autophagy activating kinase 1

Background:

Synonyms: unc-51 like autophagy activating kinase 1 , ATG1 , ATG1A , UNC51 , Unc51.1 , hATG1

Background: Involved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells.

Molecular Weight: 113 kDa

Gene ID: 8408

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy