PIK3C3 antibody (N-Term)

Catalog No. ABIN7469519

This anti-PIK3C3 antibody is a Rabbit Polyclonal antibody detecting PIK3C3 in WB and IP. Suitable for Human.

Quick Overview for PIK3C3 antibody (N-Term) (ABIN7469519)

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Grade: KO Validated

Product Details anti-PIK3C3 Antibody

Binding Specificity: N-Term

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human VPS34. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS , unboiled hVps34-transfected 293T cells Validation: KO/KD, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PIK3C3

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Alternative Name: VPS34

Background:

Synonyms: phosphatidylinositol 3-kinase catalytic subunit type 3 , VPS34 , Vps34 , hVps34

Background: Catalytic subunit of the PI3K complex. Involved in the transport of lysosomal enzyme precursors to lysosomes.

Molecular Weight: 102 kDa

Gene ID: 5289

UniProt: Q8NEB9

Pathways: AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagy