SARS-CoV-2 Spike antibody (AA 1029-1192)

Catalog No. ABIN7469592

This Mouse Monoclonal antibody specifically detects SARS-CoV-2 Spike in ELISA, WB, Neut, FACS, IF, IP, ICC, IHC (p), IEM, IHC (fp) and Inh. It exhibits reactivity toward SARS Coronavirus (SARS-CoV).

Quick Overview for SARS-CoV-2 Spike antibody (AA 1029-1192) (ABIN7469592)

Target: SARS-CoV-2 Spike

Reactivity: SARS Coronavirus (SARS-CoV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This SARS-CoV-2 Spike antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Neutralization (Neut), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoelectron Microscopy (IEM), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Inhibition (Inh)

Clone: 1A9

Product Details anti-SARS-CoV-2 Spike Antibody

Binding Specificity: AA 1029-1192

Specificity: This antibody detects both SARS-CoV spike and SARS-CoV-2 spike proteins (S2 subunit). Based on sequence analysis, this antibody is predicted to recognize S2' subunit. Our internal testing indicates no cross-reactivity with MERS-CoV spike protein.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.

Cross-Reactivity: SARS Coronavirus (SARS-CoV), SARS Coronavirus-2 (SARS-CoV-2)

Purification: Affinity purified by Protein G.

Immunogen: The immunogen used to generate this antibody corresponds to SARS-CoV Sdelta10 (within S2 domain) protein (1029-1192 a.a.). (SARS-CoV strain: Sin2774) The exact sequence is proprietary.

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Comparison, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike

Background: 2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike