CALB1 antibody (AA 7-96)

Catalog No. ABIN7477383

This Mouse Monoclonal antibody specifically detects CALB1 in IHC. It exhibits reactivity toward Human.

Quick Overview for CALB1 antibody (AA 7-96) (ABIN7477383)

Target: CALB1 (Calbindin (CALB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CALB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-471M

Product Details anti-CALB1 Antibody

Binding Specificity: AA 7-96

Immunogen: Recombinant fragment (around aa 7-96) of human CALB1 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:100

Comment:

Positive Control: Kidney: A subset of the cells of distal tubuli should show an intense cytoplasmic calbindin 1 immunostaining.

Negative Control: Colon: Calbindin 1 immunostaining should be completely absent in all cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CALB1

Target: CALB1 (Calbindin (CALB1))

Alternative Name: Calbindin 1

Background: Avian-type, CAB27, CALB 1, CALB, CALB1, CALB1_HUMAN, Calbindin 1 28 kDa, Calbindin, Calbindin D28, D 28K, D-28K, D28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL H protein, Vitamin D dependent calcium binding protein, Vitamin D dependent calcium binding protein avian type, Vitamin D-dependent calcium-binding protein,Calbindin 1 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P05937