CD31 antibody (AA 625-738)

Catalog No. ABIN7477409

The Mouse Monoclonal anti-CD31 antibody has been validated for IHC. It is suitable to detect CD31 in samples from Human.

Quick Overview for CD31 antibody (AA 625-738) (ABIN7477409)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-031M

Product Details anti-CD31 Antibody

Binding Specificity: AA 625-738

Immunogen: Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Liver: At least a weak to moderate staining of the majority of the hepatic sinusoidal endothelial cells should be seen.

Negative Control: Liver: Hepatocytes should not show any CD31 staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31

Background: EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA,CD31 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization