Recombinant CEA antibody

Catalog No. ABIN7477419

This Rabbit Monoclonal antibody specifically detects CEA in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CEA antibody (ABIN7477419)

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CEA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-465R

Product Details anti-CEA Antibody

Immunogen: Recombinant full-length human CEA protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: An at least moderate cytoplasmic CEA staining should be seen in the vast majority of columnar epithelial cells.

Negative Control: Colon: CEA staining should not be seen in stromal cells or smooth muscle.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Alternative Name: CEA

Background: Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1),CEA antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P06731