Recombinant Chromogranin A antibody

Catalog No. ABIN7477423

This anti-Chromogranin A antibody is a Rabbit Monoclonal antibody detecting Chromogranin A in IHC. Suitable for Human.

Quick Overview for Recombinant Chromogranin A antibody (ABIN7477423)

Target: Chromogranin A (CHGA)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Chromogranin A antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-380R

Product Details anti-Chromogranin A Antibody

Immunogen: Recombinant full-length human chromogranin A protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Appendix: An at least weak to moderate staining must be seen in the axons and ganglion cells of the peripheral nerves while mucosal neuroendocrine cells exhibit a strong staining (neuroendocrine cells are not sufficient as positive controls because they express CGA so strongly, that even inefficient staining will detect its expression)

Negative Control: Appendix: Epithelial and smooth muscle cells should be negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Chromogranin A

Target: Chromogranin A (CHGA)

Alternative Name: Chromogranin A

Background: Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II,Chromogranin A antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling