KRT15 antibody
Quick Overview for KRT15 antibody (ABIN7477445)
Target
See all KRT15 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Immunogen
- Recombinant full-length human KRT15 protein
-
Isotype
- IgG
-
-
-
-
Application Notes
- IHC 1:50-1:100
-
Comment
-
Positive Control: Tonsil: All cells of the surface squamous epithelium and most squamous cells of the crypts should show strong KRT15 staining.
Negative Control: Tonsil: All lymphocytes and blood vessels must not show any KRT15 staining.
-
Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- KRT15 (Keratin 15 (KRT15))
-
Alternative Name
- Cytokeratin 15
-
Background
- CK15, Cytokeratin 15, K1CO, Ka15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15,Cytokeratin 15 antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P19012
Target
-