Recombinant KRT20 antibody (AA 196-323)

Catalog No. ABIN7477451

Product Details anti-KRT20 Antibody

Target: KRT20 (Keratin 20 (KRT20))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 196-323

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Clone: MSVA-620R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon mucosa: Staining should be strong at the surface and at least moderate at the basis of krypts.

Negative Control: Tonsil: All cells must stain negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: Cytokeratin 20 (KRT20 Products)

Background: CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20,Cytokeratin 20 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P35900