GATA3 antibody
Quick Overview for GATA3 antibody (ABIN7477479)
Target
See all GATA3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Immunogen
- Recombinant full-length human GATA-3 protein
-
Isotype
- IgG
-
-
anti-GATA Binding Protein 3 (GATA3) antibody
GATA3 Reactivity: Human WB, ELISA, IHC, FACS Host: Mouse Monoclonal 1A10A11 unconjugated
anti-GATA Binding Protein 3 (GATA3) (AA 1-444) antibodyGATA3 Reactivity: Human WB, FACS, IF Host: Mouse Monoclonal 1259CT707-136-102 unconjugated
anti-GATA Binding Protein 3 (GATA3) (N-Term) antibodyGATA3 Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Sheep WB, IHC Host: Rabbit Polyclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) (AA 1-260) antibodyGATA3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) (pSer308) antibodyGATA3 Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) (AA 103-200) antibodyGATA3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 1C1 unconjugated
anti-GATA Binding Protein 3 (GATA3) (AA 103-200) antibodyGATA3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3A4 unconjugated
anti-GATA Binding Protein 3 (GATA3) (C-Term) antibodyGATA3 Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Sheep WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) antibodyGATA3 Reactivity: Human WB, IP Host: Rabbit Monoclonal unconjugated
anti-GATA Binding Protein 3 (GATA3) antibodyGATA3 Reactivity: Human ELISA, IHC Host: Mouse Monoclonal 7B5 unconjugated
-
-
Application Notes
- IHC 1:50-1:100
-
Comment
-
Positive Control: Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. , Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining.
Negative Control: Kidney: Staining should be absent in proximal and distal tubuli as well as in blood vessels. , Tonsil: Staining should be absent in B-cells.
-
Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:80 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- GATA3 (GATA Binding Protein 3 (GATA3))
-
Alternative Name
- GATA3
-
Background
- GATA3, GATA binding protein-3, GATA-binding factor 3, GATA3, HDR, HDRS, Transacting T-cell-specific transcription factor GATA-3,GATA3 antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P23771
-
Pathways
- Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
Target
-
