GLN1 antibody (AA 50-250)

Catalog No. ABIN7477483

This anti-GLN1 antibody is a Mouse Monoclonal antibody detecting GLN1 in IHC. Suitable for Human.

Quick Overview for GLN1 antibody (AA 50-250) (ABIN7477483)

Target: GLN1 (Glutamine Synthetase (GLN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLN1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-750M

Product Details anti-GLN1 Antibody

Binding Specificity: AA 50-250

Immunogen: Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Liver: A strong GS staining should be seen of centrilobular hepatocytes while staining is weak to moderate in Kupffer cells.

Negative Control: Liver: Periportal hepatocytes must be GS negative in the normal liver.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase

Background: Cell proliferation-inducing protein 59, GLUL, Glutamate ammonia ligase, GS, PIG43, PIG59, Glutamate decarboxylase, glutamine synthase, Glutamine synthetase, glutamine synthetase I, Proliferation inducing protein 43, GLNA, GLNS,Glutamine Synthetase antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion