GLN1 antibody (AA 50-250)

Catalog No. ABIN7477483

This anti-GLN1 antibody is a Mouse Monoclonal antibody detecting GLN1 in IHC. Suitable for Human.

Quick Overview for GLN1 antibody (AA 50-250) (ABIN7477483)

Target: GLN1 (Glutamine Synthetase (GLN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLN1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 750M

Product Details anti-GLN1 Antibody

Binding Specificity: AA 50-250

Purpose: Glutamine Synthetase antibody (750M)

Immunogen: Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Liver: A strong GS staining should be seen of centrilobular hepatocytes while staining is weak to moderate in Kupffer cells.

Product Description: Glutamine Synthetase antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Liver: Periportal hepatocytes must be GS negative in the normal liver.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date: 24 months

Target Details for GLN1

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase

Background: Gene Alias: cell proliferation-inducing protein 59, GLUL, Glutamate ammonia ligase, GS, PIG43, PIG59, Glutamate decarboxylase, glutamine synthase, Glutamine synthetase, glutamine synthetase I, Proliferation inducing protein 43, GLNA, GLNS

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion