Recombinant MLANA antibody

Catalog No. ABIN7477517

The Mouse Monoclonal anti-MLANA antibody has been validated for IHC. It is suitable to detect MLANA in samples from Human.

Quick Overview for Recombinant MLANA antibody (ABIN7477517)

Target: MLANA (Melan A (MLANA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-901M-

Product Details anti-MLANA Antibody

Immunogen: Recombinant full-length hMART-1 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:100

Comment:

Positive Control: Skin: Virtually all melanocytes should show a strong Melan A+ immunostaining including a weak to moderate staining in melanocytic dendrites. Adrenal gland: An at least moderate Melan A+ immunostaining should be seen in adrenocortical cells.

Negative Control: Kidney: Melan A immunostaining should be absent in all cells. Adrenal gland: Melan A+ immunostaining should be absent in all medullary cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:75 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: Melan A

Background: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA,Melan A antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q16655