Recombinant NAPSA antibody (AA 189-299)

Catalog No. ABIN7477537

This anti-NAPSA antibody is a Rabbit Monoclonal antibody detecting NAPSA in IHC. Suitable for Human.

Quick Overview for Recombinant NAPSA antibody (AA 189-299) (ABIN7477537)

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NAPSA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-112R

Product Details anti-NAPSA Antibody

Binding Specificity: AA 189-299

Immunogen: Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Kidney: an at least moderate granular cytoplasmic staining should be seen in proximal tubule cells

Negative Control: Colon: staining should be absent in epithelial and other cells of the lamina propria.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Alternative Name: Napsin A

Background: ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA,Napsin A antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: O96009

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis