Borrelia Burgdorferi antibody (FITC)

Catalog No. ABIN7478141

This Rabbit Polyclonal antibody specifically detects Borrelia Burgdorferi in Func. It exhibits reactivity toward Borrelia burgdorferi.

Quick Overview for Borrelia Burgdorferi antibody (FITC) (ABIN7478141)

Target: Borrelia Burgdorferi

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Borrelia Burgdorferi antibody is conjugated to FITC

Application: Functional Studies (Func)

Product Details anti-Borrelia Burgdorferi Antibody (FITC)

Specificity: Reactive Western Blot bands at 83kD, 41kD, 34kD and 31kD + additional low MW bands

Cross-Reactivity (Details): Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii

Purification: This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogen: Whole cell preparation from B. burgdorferi

Isotype: IgG

Application Details

Application Notes: TITER : >1:1,000 in Western Blot. >1:40,000 in avidin-biotin IFA
Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ). Functions well in formalin fixed/paraffin embedded settings

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.

Target Details for Borrelia Burgdorferi

Target: Borrelia Burgdorferi