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Diphtheria Toxin antibody (Peroxidase (POD))

The Goat Polyclonal anti-Diphtheria Toxin antibody has been validated for WB, EIA and IHC. It is suitable to detect Diphtheria Toxin in samples from Corynebacterium diphtheriae.
Catalog No. ABIN7478292

Quick Overview for Diphtheria Toxin antibody (Peroxidase (POD)) (ABIN7478292)

Target

Diphtheria Toxin

Reactivity

  • 27
  • 5
  • 1
Corynebacterium diphtheriae

Host

  • 22
  • 11
Goat

Clonality

  • 18
  • 11
Polyclonal

Conjugate

  • 12
  • 7
  • 7
  • 5
  • 1
  • 1
This Diphtheria Toxin antibody is conjugated to Peroxidase (POD)

Application

  • 20
  • 15
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (IHC)
  • Specificity

    Purified diphtheria toxin/toxoid

    Cross-Reactivity (Details)

    Demonstrated reactivity with purified toxin

    Purification

    This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

    Immunogen

    Purified diphtheria toxoid

    Isotype

    IgG
  • Application Notes

    TITER : neat in gel techniques, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
  • Target

    Diphtheria Toxin
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