This anti-Legionella Pneumophila antibody is a Rabbit Polyclonal antibody detecting Legionella Pneumophila in Func. Suitable for Legionella pneumophila.
Quick Overview for Legionella Pneumophila antibody (FITC) (ABIN7478296)
Target
Legionella Pneumophila
Reactivity
Legionella pneumophila
Host
Rabbit
Clonality
Polyclonal
Conjugate
This Legionella Pneumophila antibody is conjugated to FITC
Application
Functional Studies (Func)
Specificity
All antigens of intact microorganism
Cross-Reactivity (Details)
Antiserum is not absorbed and may crossreact with related microorganisms. Reactive with L. pneumophila serogroups 1-12 in IFA
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
Immunogen
A whole cell preparation of Legionella pneumophila
Reactivity: Legionella pneumophila
IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
TITER : > 1:800 by indirect IFA vs. acetone-fixed organsims, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.