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Legionella Pneumophila antibody (Peroxidase (POD))

This Rabbit Polyclonal antibody specifically detects Legionella Pneumophila in WB, IHC and EIA. It exhibits reactivity toward Legionella pneumophila.
Catalog No. ABIN7478297

Quick Overview for Legionella Pneumophila antibody (Peroxidase (POD)) (ABIN7478297)

Target

Legionella Pneumophila

Reactivity

  • 20
  • 5
Legionella pneumophila

Host

  • 18
  • 7
Rabbit

Clonality

  • 18
  • 7
Polyclonal

Conjugate

  • 11
  • 6
  • 5
  • 3
This Legionella Pneumophila antibody is conjugated to Peroxidase (POD)

Application

  • 13
  • 12
  • 10
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
  • Specificity

    All antigens of intact microorganism

    Cross-Reactivity (Details)

    Antiserum is not absorbed and may crossreact with related microorganisms. Reactive with L. pneumophila serogroups 1-12 in IFA

    Purification

    This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

    Immunogen

    A whole cell preparation of Legionella pneumophila

    Isotype

    IgG
  • Application Notes

    TITER : > 1:800 by indirect IFA vs. acetone-fixed organsims, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
  • Target

    Legionella Pneumophila

    Target Type

    Bacteria
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