PD-L1 antibody

Catalog No. ABIN7478848

This Rabbit Monoclonal antibody specifically detects PD-L1 in IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for PD-L1 antibody (ABIN7478848)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ZR3

Product Details anti-PD-L1 Antibody

Cross-Reactivity (Details): Human

Characteristics: Programmed cell death ligand 1 (PD-L1) also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1) is a type 1 transmembrane protein involved in the regulation of cellular and humoral immune responses. The interaction of PD-L1 with its receptor PD-1 provides both stimulatory and inhibitory signals in regulating T cell activation and tolerance during pregnancy, tissue allograft, autoimmune disease and malignant transformation. The binding of PD-L1 to PD-1 induces apoptosis or exhaustion in activated T cells, and blockade of this interaction has been shown to enhance the antitumor activity of T cells. PD-L1 is frequently over expressed in placenta, and many human tumours such as melanoma, diffuse large B-cell lymphoma, and carcinomas of the lung, bladder, breast, kidney and colon.

Purification: Antibody solution

Isotype: IgG

Application Details

Application Notes: Unmasking with Unmasking fluid E  pH 9.0 or Unmasking fluid T  pH 8.0,Placenta, lung carcinoma (NSCLC),IHC(C, P),(liquid conc.) 1:50-1:100

Restrictions: For Research Use only

Handling

Buffer: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: CD274

Background: Human CD274, PD-L1.

Pathways: Cancer Immune Checkpoints