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CD5 antibody

The Rabbit Monoclonal anti-CD5 antibody has been validated for FACS, IHC (p) and IHC (fro). It is suitable to detect CD5 in samples from Human.
Catalog No. ABIN7478849

Quick Overview for CD5 antibody (ABIN7478849)

Target

See all CD5 Antibodies
CD5

Reactivity

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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This CD5 antibody is un-conjugated

Application

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Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

EP77
  • Cross-Reactivity (Details)

    Human

    Characteristics

    The antibody to CD5 is used for the classification of lymphoid neoplasm. CD5 is exprimed by the majority of T-cell lymphoma and is also found on B-cells in some cases of chronic lymphatic leukaemia and centrocytic lymphoma. Normally it is not found in other forms of lymphoid neoplasm e.g. follicular lymphoma, Hairy Cell Lymphoma and Large Cell Lymphoma. CD5 is a lymphocyte surface molecule with a molecular weight of 67 kDa. It is exprimed by 95 % of thymocytes and app. 72 % of peripheral blood lymphocytes. In lymph nodes the antigen is detected in the T-cell region mainly. CD5 is over-expressed in leukaemia and T-cell lymphoma and may also be detected in a subpopulation of activated B-cells. Human CD5 Molecule.

    Purification

    Antibody solution

    Immunogen

    Synthetic Protein, corresponding to a sequence of human CD5 molecule

    Isotype

    IgG
  • Application Notes

    Paraffin sections are pre-treated with 1 mM EDTA ( pH 8.0) in an autoclave (2 min under full pressure, cooling 15-20 min.),Tonsil,IHC(C, P), FACS,60 min at RT,(liquid conc.) 1:10-1:25

    Restrictions

    For Research Use only
  • Buffer

    Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 μl working solution / test).

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    2-8°C
  • Target

    CD5

    Alternative Name

    CD5

    UniProt

    P06127
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