Recombinant Fc gamma RII (CD32) antibody

Catalog No. ABIN7487693

This Mouse Monoclonal antibody specifically detects in ELISA, IF, IP, BR and InhA. It exhibits reactivity toward Human.

Quick Overview for Recombinant Fc gamma RII (CD32) antibody (ABIN7487693)

Target: Fc gamma RII (CD32)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Clone: IV-3

Product Details

Purpose: Anti-CD32 [IV.3], Mouse IgG2b, kappa

Specificity: This antibody recognizes and binds CD32a.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated against the FcR of K562 cells.

Isotype: IgG2b kappa

Application Details

Application Notes: This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID: 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic ""FCGR2A"" mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32

Background: CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt: P12318