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Recombinant CD3 epsilon antibody

The Mouse Monoclonal anti-CD3 epsilon antibody has been validated for FACS and IHC. It is suitable to detect CD3 epsilon in samples from Pig.
Catalog No. ABIN7487697

Quick Overview for Recombinant CD3 epsilon antibody (ABIN7487697)

Target

See all CD3 epsilon (CD3E) Antibodies
CD3 epsilon (CD3E)

Antibody Type

Recombinant Antibody

Reactivity

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Pig

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD3 epsilon antibody is un-conjugated

Application

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Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone

898H2-6-15
  • Purpose

    Anti-CD3e [898H2-6-15], Mouse IgG2a, kappa

    Specificity

    Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically. It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 Ab (STH164). CD3, and the e chain in particular, is crucial for T cell receptor-dependent signal transduction across the lymphocyte membrane.

    Characteristics

    Original Species of Ab: Mouse

    Original Format of Ab: IgG2a

    Purification

    Protein A affinity purified

    Immunogen

    Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.

    Isotype

    IgG2a kappa
  • Application Notes

    The initial identification of 898H2-6-15 showed that it recognised the T-cell receptor-associated glycoprotein CD3, in particular the e chain (Huang et al, 1999). More recently, the 898H2-6-15 VH and VL domains were reformatted into a scFv and conjugated to diphtheria toxin (Wang et al, 2011). This version of the antibody as an 'immunotoxin' has been shown to deplete circulating T cells in swine without observation of any clinical toxicities.

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    PBS with 0.02 % Proclin 300.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target

    CD3 epsilon (CD3E)

    Alternative Name

    CD3e

    Background

    Leu4/T3, Cluster of Differentiation 3 epsilon chain, T cell glycoprotein CD3 epsilon chain

    UniProt

    Q7YRN2

    Pathways

    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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