Recombinant CD3 epsilon antibody
Quick Overview for Recombinant CD3 epsilon antibody (ABIN7487697)
Target
See all CD3 epsilon (CD3E) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Purpose
- Anti-CD3e [898H2-6-15], Mouse IgG2a, kappa
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Specificity
- Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically. It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 Ab (STH164). CD3, and the e chain in particular, is crucial for T cell receptor-dependent signal transduction across the lymphocyte membrane.
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Characteristics
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Original Species of Ab: Mouse
Original Format of Ab: IgG2a
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Purification
- Protein A affinity purified
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Immunogen
- Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.
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Isotype
- IgG2a kappa
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Application Notes
- The initial identification of 898H2-6-15 showed that it recognised the T-cell receptor-associated glycoprotein CD3, in particular the e chain (Huang et al, 1999). More recently, the 898H2-6-15 VH and VL domains were reformatted into a scFv and conjugated to diphtheria toxin (Wang et al, 2011). This version of the antibody as an 'immunotoxin' has been shown to deplete circulating T cells in swine without observation of any clinical toxicities.
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Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- PBS with 0.02 % Proclin 300.
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Preservative
- ProClin
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Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD3 epsilon (CD3E)
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Alternative Name
- CD3e
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Background
- Leu4/T3, Cluster of Differentiation 3 epsilon chain, T cell glycoprotein CD3 epsilon chain
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UniProt
- Q7YRN2
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Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
Target
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