Recombinant FCAR antibody

Catalog No. ABIN7487709

The Mouse Monoclonal anti-FCAR antibody has been validated for FACS, ELISA and IP. It is suitable to detect FCAR in samples from Human.

Quick Overview for Recombinant FCAR antibody (ABIN7487709)

Target: FCAR (Fc Fragment of IgA, Receptor For (FCAR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCAR antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP)

Clone: 14A8

Product Details anti-FCAR Antibody

Purpose: Anti-CD89 [14A8 (14.1)], Mouse IgG1, kappa

Specificity: This antibody binds CD89 in the extracellular domain 2, outside the IgA-binding site.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing HCO12 trangene containing mice with recombinant soluble CD89 antigen.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody binds human CD89 with a dissociation constant of Kd= 6.8 X10-10M.The binding of this antibody to human CD89 was confirmed with ELISA. This antibody was also capable of recognizing human CD89 expressing cells like human PMNL cells by flow cytometry. This antibody is not capable of blocking binding of IgA to CD89. This antibody can used to immunoprecipitate soluble CD89 (US7192582). A bispecific antibody construct incorporating the variable regions of the anti-gp41 antibody F240 and the anti-CD89 (immunoglobulin A [IgA] receptor) antibody 14A8 was reported to promote destruction of HIV type 1 (HIV-1) by neutrophils (PMID: 18272577). A Fab-like bispecific antibody molecule or Bi-Fab antibody generated from F240scFv and 14A8scFv showed significant antiboy dependent cell-mediated viral inhibition (ADCVI) activity for multiple clade B and clade C isolates by arming the neutrophils to inhibit HIV infection (PMID: 27419146).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for FCAR

Target: FCAR (Fc Fragment of IgA, Receptor For (FCAR))

Alternative Name: CD89

Background: Immunoglobulin alpha Fc receptor, FCAR, IgA Fc receptor, FcαR

UniProt: P24071