CD245 antibody (PE)

Catalog No. ABIN7505889

This Mouse Monoclonal antibody specifically detects in FACS. It exhibits reactivity toward Human.

Quick Overview for CD245 antibody (PE) (ABIN7505889)

Target: CD245

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS)

Clone: DY12

Product Details

Purpose: Anti-Hu CD245 PE

Specificity: The mouse monoclonal antibody DY12 recognizes an extracellular epitope of CD245, a transmembrane protein expressed mainly by peripheral blood lymphocytes, NK cells, monocytes, granulocytes and platelets.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: human NK-type YT2C2 cells

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details

Target: CD245

Background: CD245 is a 220-250 kDa transmembrane protein of unknown structure. It is expressed at lower level on resting blood lymphocytes, granulocytes, platelets, and thymocytes, and at high level on monocytes and on IL-2-dependent T cell lines. It is also expressed on Ewing sarcoma and osteosarcoma cell lines. CD245 seems to act as a costimulation molecule of T and NK cells.,p220/240, DY12, DY35