N-Cadherin antibody (Extracellular Domain) (APC)

Catalog No. ABIN7505915

This anti-N-Cadherin antibody is a Mouse Monoclonal antibody detecting N-Cadherin in FACS. Suitable for Human and Mouse.

Quick Overview for N-Cadherin antibody (Extracellular Domain) (APC) (ABIN7505915)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: 8C11

Product Details anti-N-Cadherin Antibody (APC)

Binding Specificity: Extracellular Domain

Purpose: Anti-CD325 APC

Specificity: The mouse monoclonal antibody 8C11 recognizes an extracellular epitope of CD325 (N-cadherin), a transmembrane protein associated with actin cytoskeleton system, which is expressed mainly by neurons, osteoblasts, endothelial cells, and stem cells.

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: bacterially expressed extracellular domain of human CD325

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CD325

Background: Cadherin 2,CD325 (N-cadherin) is a type I transmembrane protein, which forms a complex with catenins, that is linked to the actin cytoskeleton. This complex is important in synapses and for functional plasticity of neurons, and is also essential for embryonic development. Decreased CD325 cleavage caused by mutations in presenilin 1 is associated with Alzheimer´s disease. Besides nervous system, CD325 is expressed on the surface of malignant T cells, and increases their adhesion to epithelia, as well as their ability to invade and metastasize to inflammatory sites.,N-Cadherin, cadherin 2, CDHM, CDH2

Gene ID: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane