IgE antibody (APC)

Catalog No. ABIN7505983

The Mouse Monoclonal anti-IgE antibody has been validated for FACS. It is suitable to detect IgE in samples from Human.

Quick Overview for IgE antibody (APC) (ABIN7505983)

Target: IgE

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgE antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: 4H10

Product Details anti-IgE Antibody (APC)

Purpose: Anti-Hu IgE APC

Specificity: The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7.325 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Purified human IgE.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 0.5-3 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for IgE

Target: IgE

Background: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E