BIP/GRP78 antibody
Quick Overview for BIP/GRP78 antibody (ABIN7536640)
Target
See all BIP/GRP78 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purpose
- GRP78 Polyclonal Antibody
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Specificity
- Human GRP78 (HSPA5) (Heat Shock 70 kDa Protein 5 (Glucose-Regulated Protein, 78 kDa)
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Purification
- Antigen-specific affinity purification
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Immunogen
- Recombinant human GRP78
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Isotype
- IgG
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Application Notes
- Immunoblot 0.5-5 μg/mL, ELISA capture 10 μg/mL
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Comment
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Affinity-purified IgG are antibodies further purified using immobilized antigen. Affinity-purification is an enrichment process in which whole IgG is passed over a resin to which antigen has been covalently attached. Non-reactive antibodies are washed away and specific antibodies are eluted under conditions that disrupt the antigen-antibody interaction. The result is a very high-purity product with a substantially higher titre than whole IgG. This level of product is intended for applications such as immunoblotting, immunostaining of cells and several types of immunoassays where the higher signal-to-noise ratio achieved with this enriched product is required.
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Allow product to warm to room temperature and gently mix before use
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Concentration
- 1 mg/mL
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Buffer
- 10 mM HEPES, pH 7.4, 150 mM NaC, 50 % (v/v) glycerol
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Storage
- -20 °C
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Storage Comment
- Store between -10°C and -20°C
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- BIP/GRP78 (Luminal-Binding Protein (BIP/GRP78))
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Alternative Name
- Glucose-Regulated Protein 78 (GRP78)
Target
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