Dystroglycan antibody (C-Term)

Catalog No. ABIN7538842

This Mouse Monoclonal antibody specifically detects Dystroglycan in IHC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Dystroglycan antibody (C-Term) (ABIN7538842)

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dystroglycan antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 43DAG1-8D5

Product Details anti-Dystroglycan Antibody

Binding Specificity: C-Term

Purpose: Lyophilized Mouse Monoclonal Antibody Beta-Dystroglycan

Specificity: Human beta-dystroglycan (43 kD). Also crossreacts strongly with beta-dystroglycan in sections of mouse, rat, rabbit, dog and chicken, hamster and toad muscle. Other animal species not tested.

Characteristics: This product is intended for the qualitative identification by light microscopy of Beta-Dystroglycan by immunohistochemistry.

Purification: Tissue culture supernatant

Immunogen: Synthetic peptide containing 15 of the last 16 amino acids at the extreme C-terminus of the human beta-dystroglycan sequence (PKNMTPYRSPPPYVP-PCOOH).

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on frozen sections. Suggested dilution: 1:50-1:200 for 60 minutes at 25°C. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.

Concentration: 240 mg/L

Buffer: This product is a lyophilized tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Alternative Name: Beta-Dystroglycan

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus