Recombinant CD31 antibody (Extracellular Domain) (Atto 647N)

Catalog No. ABIN7540331

This anti-CD31 antibody is a Alpaca Multiclonal antibody detecting CD31 in IF, IF/ICC and IF (p). Suitable for Mouse.

Quick Overview for Recombinant CD31 antibody (Extracellular Domain) (Atto 647N) (ABIN7540331)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Mouse

Host: Alpaca

Clonality: Multiclonal

Conjugate: This CD31 antibody is conjugated to Atto 647N

Application: Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 3ELC120-3ELC140-3ELC105-3ELC143

Product Details anti-CD31 Antibody (Atto 647N)

Binding Specificity: Extracellular Domain

Expression System: E.coli

Purpose: Alpaca anti-mouse CD31 VHH is a carefully developed and validated nanobody (single-domain antibody) binding to human CD31 (PECAM-1) highly suitabe for tissue staining.

Brand: LIMAAbody®

Specificity: Detects endogenous levels of total murine CD31 protein.

Cross-Reactivity (Details): not analysed

Characteristics: VHH protein tag: C-terminal (6x) His-Tag

Purification: Affinity-purified antibody fragment.

Immunogen: Recombinant protein containing the extracellular domain of murine CD31 protein.

Application Details

Application Notes: Immunofluorescence: 10 μg/mL (frozen sections), 1 μg/mL (cell culture), 20 μg/mL (wholemount)

Comment:

VHH protein tag: C-terminal (6x) His-Tag

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Upon receipt store at 4°C. Stable for 6 months. Do not freeze.

Expiry Date: 6 months

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31

Background: CD31, also known as PECAM-1, is an adhesion molecule and is expressed on endothelial cells at intercellular junctions and various T cell subsets. It shows altered expression levels in arterial, venous and lymphatic vessels. It is also found to a lesser extent on platelets, as well as most other leukocytes including monocytes and neutrophils. CD31 facilitates homotypic binding to itself, as well as heterotypic binding to the leukocyte integrin alpha V beta III. CD31 plays a crucial role in facilitating the transendothelial migration of leukocytes through the intercellular junctions of vascular endothelial cells. It is one of key regulatory molecules in vascular system.

Molecular Weight: 81 kDa

NCBI Accession: NP_033868

UniProt: Q08481

Pathways: Regulation of Actin Filament Polymerization