SOD3 antibody

Catalog No. ABIN7540479

Supplier Product No.: 200-301-f24

The Mouse Monoclonal anti-SOD3 antibody has been validated for WB, IHC, ELISA, IF and FM. It is suitable to detect SOD3 in samples from Human.

Quick Overview for SOD3 antibody (ABIN7540479)

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 4GG11G6

Product Details anti-SOD3 Antibody

Supplier Product No.: 200-301-f24

Supplier: Rockland

Purpose: SOD3 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with SOD3 from human, rat, and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-SOD3 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: SOD3 Antibody was produced in mice by repeated immunizations with human SOD3 purified from aortas.

Isotype: IgG1 kappa

Application Details

Application Notes:

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-SOD3 Antibody has been tested by WB, IHC and IF and is recommended for use in ELISA. Expect a band approximately ~35kDa in specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SOD3

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Alternative Name: SOD3

Background: EC-SOD, MGC20077, EC 1.15.1.1, SOD3, Extracellular superoxide dismutase [Cu-Zn],Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. In general, SODs play a major role in antioxidant defense mechanisms. There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity.

Gene ID: 6649

NCBI Accession: NP_003093

UniProt: P08294