KCNMA1 antibody (C-Term)

Catalog No. ABIN7540490

Supplier Product No.: 200-301-g15

Product Details anti-KCNMA1 Antibody

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-g15

Supplier: Rockland

Purpose: mSlo maxi- K+ channel Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Slo from Mouse, Human, Rat, and Zebrafish based on 100 % homology with the immunizing sequence.

Purification: Anti-mSlo maxi- K+ channel Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: mSlo maxi- K+ channel Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to the cytoplasmic C-terminus region of mouse Slo1.

Clone: S6-60

Isotype: IgG2a

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-mSlo maxi- K+ channel Antibody is tested for use in WB, IP, and IHC. Expect a band approximately ~110-130kDa on specific lysates. Epitope mapped within amino acids 690-715. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for KCNMA1

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Alternative Name: Kcnma1 (KCNMA1 Products)

Background: Slo, BKCa, Slo1, mSlo, MaxiK, mSlo1, 5730414M22Rik, Kcnma1, Calcium-activated potassium channel subunit alpha-1, BK channel, BKCA alpha, Calcium-activated potassium channel, subfamily M subunit alpha-1, K(VCA)alpha, KCa1.1, Maxi K channel, Slo-alpha, Slo1,The Slo1 channel is a novel potassium channel that is regulated by both Ca2+ and voltage. It is a homolog to the pH and voltage-regulated Slo3 channel. Four distinct subunits composing the KCNMB family are known to regulate the function and expression of Slo1 channels. Slo1 has been shown to be involved in neurotransmitter release and etheanol response. It has been reported that SLO1 also has a critical role is muscles- the inactivation of the slo-1 gene in muscles leased to phenotypes similar to those cause by mutations of the dystrophin homologue dys-1.

Gene ID: 16531

NCBI Accession: NP_034740

UniProt: Q08460

Pathways: Regulation of Hormone Metabolic Process, Sensory Perception of Sound