KCNMA1 antibody (Internal Region)

Catalog No. ABIN1048988

Product Details anti-KCNMA1 Antibody

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Cow, Zebrafish (Danio rerio), Rabbit, Dog, Xenopus laevis, Chicken, Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNMA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human KCNMA1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Bovine, Dog, Rabbit, Pig, Turkey, Chicken, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from internal region of human KCNMA1. Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Hamster, Bovine, Dog, Rabbit, Pig, Turkey, Chicken, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KCNMA1

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Alternative Name: KCNMA1 / BK (KCNMA1 Products)

Synonyms: BKTM antibody, KCa1.1 antibody, MaxiK antibody, SAKCA antibody, SLO antibody, SLO-ALPHA antibody, SLO1 antibody, bA205K10.1 antibody, mSLO1 antibody, DDBDRAFT_0190653 antibody, DDBDRAFT_0235401 antibody, DDB_0190653 antibody, DDB_0235401 antibody, KCNMA antibody, KCNMA1 antibody, 5730414M22Rik antibody, BKCa antibody, Slo antibody, Slo1 antibody, mSlo antibody, mSlo1 antibody, KCNMA1b antibody, KCNMA1c antibody, Kcnma antibody, bktm antibody, kcnma1-A antibody, sakca antibody, cSlo antibody, slo1 antibody, kcnma1 antibody, si:ch211-39f22.2 antibody, BcDNA:GH10751 antibody, CG10693 antibody, Dmel\CG10693 antibody, Dslo antibody, dSlo antibody, dSlo1 antibody, dslo antibody, fSlo antibody, potassium calcium-activated channel subfamily M alpha 1 antibody, calcium-activated BK potassium channel, alpha subunit antibody, potassium large conductance calcium-activated channel, subfamily M, alpha member 1 antibody, potassium calcium-activated channel subfamily M alpha 1 L homeolog antibody, potassium large conductance calcium-activated channel, subfamily M, alpha member 1a antibody, calcium-activated potassium channel subunit alpha-1 antibody, slowpoke antibody, KCNMA1 antibody, kcnma1 antibody, Kcnma1 antibody, kcnma1.L antibody, kcnma1a antibody, LOC100454095 antibody, slo antibody

Background: Name/Gene ID: KCNMA1
Subfamily: Potassium channel - calcium-activated
Family: Ion Channel

Synonyms: KCNMA1, BA205K10.1, BKCA alpha subunit, BK channel, BKCA alpha, BKTM, HSlo, K(VCA)alpha, KCa1.1, Maxi-K channel HSLO, MaxiK, KCNMA, SAKCA, SLO-ALPHA, Slowpoke homolog, Stretch-activated Kca channel, Slo homolog, BK channel alpha subunit, Maxi K channel, MSlo, MSLO1, SLO, SLO1

Gene ID: 3778

Pathways: Regulation of Hormone Metabolic Process, Sensory Perception of Sound