5-mC antibody

Catalog No. ABIN7540498

Supplier Product No.: 200-301-u98

This anti- antibody is a Mouse Monoclonal antibody detecting in DB, FM, IF and IP. Suitable for Human.

Quick Overview for 5-mC antibody (ABIN7540498)

Target: 5-mC

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Fluorescence Microscopy (FM), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details

Supplier Product No.: 200-301-u98

Supplier: Rockland

Purpose: 5-mC Antibody

Cross-Reactivity (Details): Cross-reactivity with 5-mC from other sources has not been determined.

Purification: Anti-5-mC Antibody was purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen: Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methyl Cytidine.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: 1-2 μg/IP

IF_Microscopy_Dilution: 1:500

Other: FISH dilution: 1:200 - 1:1,000. Dot Blot: 1:600

Comment:

Suggested Applications: FISH
Anti-5-mC Antibody is tested for IF and Dot Blotting, Fluorescence In Situ Hybridization (FISH), Immunofluorescence and Methylated DNA Immunoprecipitation (MeDIP). Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: 5-mC

Background: 5-methylcytidine, 5-methyl Cytidine,5-methyl Cytidine is a modified nucleotide found in the DNA of both plants and vertebrates. Anti-5-mC Antibody is ideal for research in Epigenetics, specifically DNA methylation, due to its applicability to methylation analysis.